*617867
Table of Contents
▼External Links
► Genome
► Protein
►Gene Info
►Clinical Resources
▼ Variation
►Animal Models
►Cellular Pathways
* 617867
TUMOR PROTEIN p53-INDUCIBLE PROTEIN 11; TP53I11
Alternative titles; symbols
p53-INDUCIBLE GENE 11; PIG11
HGNC Approved Gene Symbol: TP53I11
Cytogenetic location: 11p11.2 Genomic coordinates (GRCh38): 11:44,932,348-44,951,306 (from NCBI)
TEXT
▼ Description
TP53I11 is induced by p53 (TP53; 191170) and is predicted to play a role in p53-mediated apoptosis (Liang et al., 2004).
▼ Cloning and Expression
Using serial analysis of gene expression (SAGE) to evaluate changes in gene expression in DLD-1 colorectal cancer cells transfected with p53, Polyak et al. (1997) identified PT53I11, which they called PIG11. Northern blot analysis detected a 2.3-kb PIG11 transcript in DLD-1 cells following p53 exposure. By RT-PCR of MGC-803 human gastric carcinoma cell total RNA, Liang et al. (2004) cloned PIG11. The deduced 121-amino acid protein has a calculated molecular mass of 12.9 kD. Fluorescence-tagged PIG11 localized to a web of cytoplasmic filaments. Xiong et al. (2007) found that PIG11 predominantly localized to cytoplasm of transfected HEK293 cells, but that a proportion localized to nuclei in other transfected cells.
▼ Gene Function
Liang et al. (2004) found that PIG11 had weak apoptotic activity following overexpression in HEK293 cells. PIG11 increased generation of reactive oxygen species and enhanced apoptosis induced by arsenic trioxide. Using a gel retardation assay in vitro, Xiong et al. (2007) found that recombinant human PIG11 bound to supercoiled and relaxed closed circular plasmid DNA or DNA with different length. Binding was independent of DNA sequence. Changing salt concentration and introduction of a reducing agent revealed that binding was influenced by charge effects and reduction of cysteine residues in PIG11. Atomic force microscopy revealed assembly of PIG11 protein into particles along DNA that resulted in a beads-on-a-string appearance. Each PIG11 particle appeared to bind 15 bp of DNA.
▼ Gene Structure
Liang et al. (2004) determined that TP53I11 is a single-exon gene. They identified a consensus TP53-binding site in the TP53I11 promoter.
▼ Mapping
▼ REFERENCES
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Liang, X.-Q., Cao, E.-H., Zhang, Y., Qin, J.-F. A P53 target gene, PIG11, contributes to chemosensitivity of cells to arsenic trioxide. FEBS Lett. 569: 94-98, 2004. [PubMed: 15225615, related citations] [Full Text]
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Polyak, K., Xia, Y., Zweier, J. L., Kinzler, K. W., Vogelstein, B. A model for p53-induced apoptosis. Nature 389: 300-305, 1997. [PubMed: 9305847, related citations] [Full Text]
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Xiong, X.-F., Li, H., Cao, E.-H. PIG11 protein binds to DNA in sequence-independent manner in vitro. Biochem. Biophys. Res. Commun. 358: 29-34, 2007. [PubMed: 17482569, related citations] [Full Text]
Creation Date:
Patricia A. Hartz : 02/06/2018
Edit History:
mgross : 02/06/2018
* 617867
TUMOR PROTEIN p53-INDUCIBLE PROTEIN 11; TP53I11
Alternative titles; symbols
p53-INDUCIBLE GENE 11; PIG11
HGNC Approved Gene Symbol: TP53I11
Cytogenetic location: 11p11.2 Genomic coordinates (GRCh38): 11:44,932,348-44,951,306 (from NCBI)
TEXT
Description
TP53I11 is induced by p53 (TP53; 191170) and is predicted to play a role in p53-mediated apoptosis (Liang et al., 2004).
Cloning and Expression
Using serial analysis of gene expression (SAGE) to evaluate changes in gene expression in DLD-1 colorectal cancer cells transfected with p53, Polyak et al. (1997) identified PT53I11, which they called PIG11. Northern blot analysis detected a 2.3-kb PIG11 transcript in DLD-1 cells following p53 exposure. By RT-PCR of MGC-803 human gastric carcinoma cell total RNA, Liang et al. (2004) cloned PIG11. The deduced 121-amino acid protein has a calculated molecular mass of 12.9 kD. Fluorescence-tagged PIG11 localized to a web of cytoplasmic filaments. Xiong et al. (2007) found that PIG11 predominantly localized to cytoplasm of transfected HEK293 cells, but that a proportion localized to nuclei in other transfected cells.
Gene Function
Liang et al. (2004) found that PIG11 had weak apoptotic activity following overexpression in HEK293 cells. PIG11 increased generation of reactive oxygen species and enhanced apoptosis induced by arsenic trioxide. Using a gel retardation assay in vitro, Xiong et al. (2007) found that recombinant human PIG11 bound to supercoiled and relaxed closed circular plasmid DNA or DNA with different length. Binding was independent of DNA sequence. Changing salt concentration and introduction of a reducing agent revealed that binding was influenced by charge effects and reduction of cysteine residues in PIG11. Atomic force microscopy revealed assembly of PIG11 protein into particles along DNA that resulted in a beads-on-a-string appearance. Each PIG11 particle appeared to bind 15 bp of DNA.
Gene Structure
Liang et al. (2004) determined that TP53I11 is a single-exon gene. They identified a consensus TP53-binding site in the TP53I11 promoter.
Mapping
Liang et al. (2004) stated that the TP53I11 gene maps to chromosome 11p11.2.
REFERENCES
-
Liang, X.-Q., Cao, E.-H., Zhang, Y., Qin, J.-F. A P53 target gene, PIG11, contributes to chemosensitivity of cells to arsenic trioxide. FEBS Lett. 569: 94-98, 2004. [PubMed: 15225615] [Full Text: https://doi.org/10.1016/j.febslet.2004.05.057]
-
Polyak, K., Xia, Y., Zweier, J. L., Kinzler, K. W., Vogelstein, B. A model for p53-induced apoptosis. Nature 389: 300-305, 1997. [PubMed: 9305847] [Full Text: https://doi.org/10.1038/38525]
-
Xiong, X.-F., Li, H., Cao, E.-H. PIG11 protein binds to DNA in sequence-independent manner in vitro. Biochem. Biophys. Res. Commun. 358: 29-34, 2007. [PubMed: 17482569] [Full Text: https://doi.org/10.1016/j.bbrc.2007.04.048]
Creation Date:
Patricia A. Hartz : 02/06/2018
Edit History:
mgross : 02/06/2018
